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Y-Age Clinical Studies

Glutathione Patches improve Cellular Physiologic Functiona Status in Different Organs

By Sherry Blake-Greenberg ND, MA, HMD, and Homer Nazeran PhD, CPEng (Biomed.)
Health Integration Therapy, Palos Verdes Estates, California 90274, USA
*Electrical and Computer Engineering, University of Texas at El Paso, El Paso Texas 79968, USA

Abstract:

Glutathione, termed the “ultimate” or “master” antioxidant, is a vital intracellular tripeptide molecule and plays a central role in cellular physiologic functions. It is very important in cellular detoxification and protection from damage caused by free radicals, peroxides and toxins. Blood glutathione levels are indicative of overall health. Glutathione metabolism and its cause and effect relationship to diseases such as cancer, neurodegenerative diseases, cystic fibrosis, HIV, aging and others have been shown in biomedical literature. Currently the undeniable connection between glutathione and good health is very well established.

Bioelectrical impedance data indicative of cellular physiologic organ function (status), using an Electro Interstitial Scanning (EIS) system, were acquired from two cohort volunteers after giving informed consent. Cohort 1 comprised of 10 subjects: 1 male and 9 females, 18-86 years of age while Cohort 2 were 20 subjects: 4 males and 16 females, 18-86 years of age. Cellular physiologic function in subjects were evaluated in 8 organs (pancreas, liver, gall bladder, intestines, left and right adrenal glands, hypothalamus and pituitary gland) while wearing the glutathione patch for a period of 4 weeks. Physiologic function testing was repeated each week. Cohort 1 wore the glutathione patch for 12 hours/day daily, while Cohort 2 wore the glutathione patch for 12 hours/day on weekdays.

Cellular physiologic function baseline data were acquired from all subjects at the beginning of the study period before if Ive had a facelift. - Chuck M. the glutathione patch was worn. Subjects were instructed to keep well hydrated during the study period. All subjects served as their own control. The hypothesis to be tested was: The glutathione patch worn 12 hours daily for four weeks significantly improves cellular physiologic functional status in different organs. Statistical analyses were carried out in both cohorts comparing the cumulative averages of the net changes in cellular physiologic functional status of each organ at the end of the study period with corresponding baseline data. The results in Cohort 1 showed a highly significant (p < 0.001) improvement in physiologic functional status of all organs tested except in pancreas that showed a very significant improvement (p < 0.01). Average statistical power considering the effect size (% improvement in physiologic function, sample number, and level of significance) was at least 72% in Cohort1. The results in Cohort 2 showed a significant (p < 0.05) improvement in physiologic functional status of fours organs (adrenal glands, hypothalamus and pituitary gland). Average statistical power considering the effect size (% improvement in physiologic function, sample number, and level of significance) for these organs was at least 76% in these tests. No significance improvement in cellular physiologic status was observed in pancreas, liver, gall bladder and intestines in Cohort 2. This could be attributed to the fact that by not using the patches for 2 days in a week (about 30% less exposure to glutathione), the subjects in Cohort 2 did not achieve adequate stimulated detoxification in all organs by glutathione over the study period.

The overall data in Cohort 1 in this study demonstrated that glutathione patches worn 12 hours daily over a period of 4 weeks produced a highly significant improvement in physiologic functional status of pancreas, liver, gall bladder, intestines, left and right adrenals, hypothalamus and pituitary gland and very significant improvement in pancreas with a statistical power of at least 72%. Stated differently all organs achieved significant cellular physiologic functional status improvement compared to baseline with a statistical power of at least 91%.

Introduction:

Glutathione is a vital intracellular tripeptide molecule comprised of 3 nonessential amino acids: cysteine, glutamic acid and glycine (g-glutamyl-cysteinyl-glycine abbreviated as GSH). These 3 building blocks in turn are made from different combinations of essential amino acids. The –SH suffix in GSH (reduced form of glutathione) indicates that it contains a sulfhydryl group. This group comes from sulfurcontaining amino acids cysteine and methionine. Glutathione is produced naturally in abundance in the body and circulates constantly in the bloodstream neutralizing free radicals (dangerous by-products of normal metabolic processes converting food to energy) and removing environmental poisons such as heavy metals, harmful waste products and toxins to protect cells against oxidative stress. Free radicals are unstable oxygen-containing molecules which are hungry for electrons to quench their insatiable desire for cell destruction. Glutathione is a powerful antioxidant (created by the same energy-producing processes that create free radicals) which serves as a built-in defense against the harmful effects of free radicals, by rapidly quenching the destructive free electrons in these molecules. The balancing act between free radicals and antioxidants could be easily disrupted for any reason such as when the body is under stress, fighting an infection or inflammation or healing from an injury, in which case more free radicals are generated. Free radicals are also created when the body is exposed to cigarette smoke, alcohol, ultraviolet light, heavy metals, air pollution, pesticides, food additives, and other environmental toxins. Free radicals are the underlying cause of a variety of illnesses in the body [1].

Lyons et al, described that glutathione serves diverse physiologic functions such as detoxification of xenobiotics, protection of cells from oxidative stress, and acts as a storage and a transport form of cysteine. They explained that reduced tissue levels of GSH are thought to compromise cell function, promote tissue damage, and increase morbidity under various disease conditions [2].

Wu et al, studied glutathione metabolism and its implications for health. They described that glutathione plays important roles in antioxidant defense, nutrient metabolism, and regulation of a variety of cellular events. They also explained that glutathione deficiency contributes to oxidative stress playing a key role in aging and the pathogenesis of many diseases. These diseases include: seizure, Alzheimer’s, Parkinson’s, liver disease, cystic fibrosis, sickle cell anemia, human immunodeficiency virus (HIV), acquired immunodeficiency syndrome (AIDS), cancer, heart attack, stroke, and diabetes. They emphasized the need for new understanding of the nutritional regulation of GSH metabolism as a critical step for development of effective health improvement and disease treatment strategies [3].

Townsend et al, provided an overview of the biological importance of GSH at cellular and organism level and showed cause and effect relationships between GSH metabolism and diseases such as cancer, neurodegenerative diseases, cystic fibrosis (CF), HIV, and aging. They also showed how the enzymes involved in GSH regulation and control influence susceptibility and progression of these conditions. They concluded that there seemed to be no harm in supplementing a diet with GSH as “perhaps the product will provide a supply of the constituent amino acids, where, in particular, cysteine may be useful in stimulating gastrointestinal synthesis of GSH” [4].

The current methods of oral supplementation with glutathione or its amino acid precursors have not been effective in significantly elevating the blood levels of this antioxidant due to stomach acid destruction of L-Glutathione and unpredictability of results with precursor amino acids. Direct daily injection of glutathione has been more effective in producing short term elevation of glutathione, however this approach is unreliable due to expense and inconvenience. Preliminary clinical data from blood and urine samples collected every 24 hours over a period of 5 days from 15 volunteers wearing the glutathione patch have shown a 3 to 4 fold increase in blood levels of glutathione compared to baseline levels [5].

This is the first study of its kind to investigate the effect of the glutathione patch on organ physiologic function. Bioelectrical impedance data indicative of cellular physiologic function, using and EIS system, were acquired from two cohort volunteers. Cohort 1 comprised of 10 subjects: 1 male and 9 females, 18-86 years of age while Cohort 2 comprised of 20 subjects: 4 males and 16 females, 18-86 years of age. Cellular physiologic function in subjects were evaluated in 8 organs (pancreas, liver, gall bladder, intestines, left and right adrenal glands, hypothalamus and pituitary gland) while wearing the glutathione patch for a period of 4 weeks. Physiologic function testing was repeated each week. Each visit was approximately 1 hour in duration for the testing. Cohort 1 wore the glutathione patch for 12 hours daily, while Cohort 2 wore the patch for 12 hours/day on weekdays only. Physiologic function baseline data were acquired from all subjects at the beginning of the study period before the glutathione patch was worn. Subjects were instructed to keep well hydrated during the study period. All subjects served as their own control. The overall data in this study demonstrated that glutathione patches worn 12 hours daily over a period of 4 weeks caused highly significant improvement in physiologic functional status of pancreas, liver, gall bladder, intestines, left and right adrenals, hypothalamus and pituitary gland and very significant improvement in pancreas with a statistical power of at least 72%. Stated differently all organs achieved significant cellular physiologic functional improvement with a statistical power of at least 91%.

Materials and Methods:

For this investigation, the glutathione patch (LifeWave, La Jolla, California, USA) was used. The glutathione patch is described as “a new method for increasing glutathione levels by stimulating acupuncture points on the body with a combination of pressure and infrared energy. The LifeWave glutathione patch is a non-transdermal patch that does not put any chemicals or drugs into the body. The LifeWave glutathione patch contains natural nontoxic crystals that absorb body heat to generate infrared signals that cause the body to produce more endemic glutathione. Clinical studies utilizing blood analyses indicate an average rise of more than triple the blood glutathione over a period of 24 hours” [5]. For a comprehensive discussion of the LifeWave glutathione patch please see reference [6].

An EIS (Electro Interstitial Scan, U.S. patent No. US 61/194,509) system was deployed to acquire bioelectrical impedance data indicative of cellular physiologic functional status in 8 organs. “The EIS provides an electrical signal corresponding to the status of a patient's physiological parameters: Na+/K+ATPase pump activity, tissue pCO2, sympathetic system activity and microcirculation blood flow.” [7]. The EIS System uses chronoamperometry based on Cottrel’s equation [8]. It is based on bioelectrical impedance and physiology of the interstitial fluid. It introduces low intensity direct currents at 1.2 V into the body to measure only one compartment of the interstitial fluid. “The EIS System with world wide patents (No 06/09878 and 065217) is the only commercially available device utilizing a Direct Current, allowing in vivo analysis of the physiological parameters at the cellular level via the
interstitial fluid. The 3 minute test is free of any operator bias. The EIS system using Chronoamperometry, models human body systems with measurements of physiological data.” [9].

The EIS is a hardware/software computerized system that applies precise algorithms and proprietary formulas to generate on-screen, 3-D modeling representations of the human body's systems; with specific intended uses. EIS system is a French electrochemical device, classified as a medical device in Europe and the United States. Its main functions are to read the different processes going on in the body, hyper-activity and hypo-activity in the organs. EIS measures the biochemistry and hormone levels.

It also measures pH, body composition and the sympathetic and parasympathetic system. Even emotional traumas can be detected by measuring the biochemistry and cellular activity in various areas of the brain. It is measuring by sending harmless, low voltage frequencies to and from 6 electrodes connected to the body. The computer software calculates everything based on the changes made to these signals on their path through the body. Most measurements are done based on the extracellular fluids, which is the environment of all cells. This is where the biochemistry is most important, and where cellular activity can be measured by looking at what goes into and out of the cells. EIS scans the whole body in 3 minutes. It is a biofeedback device in the United States with pending FDA approval.

Inclusion criteria for participation in this study were functional individuals who were willing to wear the glutathione patch and participate in the study for a period of four weeks. Participants also agreed to not commence with any other new therapy or methods of healing and/or make any major changes in their daily life that could alter the efficacy of the study. Subjects must not have worn the glutathione patch prior to the study. Subjects were recruited from the local area of Palos Verdes and may or may not have been previous patients of Health Integration Therapy. Two cohort of volunteer subjects participated in this study. Cohort 1 comprised of 10 subjects: 1 male and 9 females, 18-86 years old while Cohort 2 were 20 subjects: 4 males and 16 females, 18-86 years old. Cohort 1 wore the glutathione patch for 12 hours daily, while Cohort 2 wore the glutathione patch for 12 hours/day on weekdays only. After giving informed consent, cellular physiologic function baseline data were acquired from all subjects at the beginning of the study period before the glutathione patch was worn and weekly afterwards for 4 weeks. Subjects were instructed to keep well hydrated during the study period. All subjects served as their own control. The subjects were instructed to place the glutathione patch 2 inches inferior to the navel (below belly button) or on CV6. Figure 1 shows the glutathione patch and figure 2 shows the anatomical position for wearing the glutathione patch.

Figure 1. The LifeWave glutathione patch.
Figure 2. The anatomical position for wearing the glutathione patch (CV6).

Results:

The Electro Interstitial Scan (EIS) System used in this investigation measured cellular physiologic function on a scale of -100 to 0 for under-function and 0 to +100 for over-function. A reading in the -20 to + 20 is range indicative of normal values for organ function.

Table 1 shows typical EIS System readings (cellular function physiologic status) for a female subject, while Table 2 shows typical EIS System readings for a male subject as examples. Functional status changes from week to week are noted as D1, D2, D3 and D4, for the 4-week period showing cellular physiologic changes in the organs. Davg shows the average value of changes for the 4-week period, and D total represents the average total physiologic change after 4 weeks with respect to baseline readings. Table 3 shows the overall mean values and standard deviations for baseline and total change in physiologic function for each of the organs in Cohort 1 (n =10).

Table 1. Typical Electro Interstitial Scan (cellular function physiologic status) data for a female subject in Cohort 1.
ORGAN NAME
Date	Panc.	Liver	Gall	Intest.	RAdren.	LAdren.	Hyp.	Pit.
Baseline	-70	-73	-73	-72	-65	-70	-46	-2
Week 1	-42	-43	-43	-49	-34	-32	-22	0
Week 2	-38	-52	-52	-29	-27	-26	-25	0
Week 3	-58	-64	-64	-66	-49	-47	-27	0
Week 4	-55	-61	-61	-58	-32	-29	-26	0

DDDD1	28	30	30	23	31	38	24	2
DDDD2	4	-9	-9	20	7	6	-3	0
DDDD3	-20	-12	-12	-37	-22	-21	-2	0
DDDD4	3	3	3	8	17	18	1	0
DDDDT	15	12	12	14	33	41	20	2
DDDDT-base	85	85	85	86	98	111	66	4

Table 2. Typical Electro Interstitial Scan (cellular function physiologic status) data for a male subject in Cohort 1.
ORGAN NAME

Date	Panc.	Liver	Gall	Intest.	RAdren.	LAdren.	Hyp.	Pit.
Baseline	-6	-7	-7	-19	-32	-34	-15	-2
Week 1	16	2	2	27	-47	-45	-29	-21
Week 2	23	6	6	31	-43	-48	-26	-21
Week 3	32	0	0	28	-20	-21	-13	1
Week 4	22	14	14	21	14	-13	2	1

DDDD1	22	-5	-5	46	-15	-11	-14	-19
DDDD2	7	4	4	4	4	-3	3	0
DDDD3	9	-6	-6	-3	23	27	13	22
DDDD4	-10	14	14	-7	34	8	15	0
DDDDT	28	7	7	40	46	21	17	3
DDDDT-base	34	14	14	59	78	55	32	5

Table 3. Summary of mean and standard deviation values for EIS readings in 8 organs in Cohort 1, n = 10.
ORGAN NAME

	Panc.	Liver	Gall	Intest.	RAdren.	LAdren.	Hyp.	Pit.
Avg Baseline	-24.2	-30.9	-30.9	-18.6	-26.8	-30.1	-21.5	-0.8
Avg DTotal	18.4	31	31	25	35.6	34.5	27.8	1.1
Avg Std Baseline	18.0	19.6	19.6	32.2	22.4	16.6	12.9	1.3
Avg Std DTotal	29.0	27.1	27.1	38.8	52.2	39.2	22.3	2.64

Discussion and Conclusion:

Statistical analyses were carried out in both cohorts comparing the cumulative averages of the net changes in physiologic functional status of each organ at the end of the study period with corresponding baseline data. The results in Cohort 1 showed a highly significant (p < 0.001) improvement in physiologic functional status of all organs tested except in pancreas that showed a very significant improvement (p < 0.01). Average statistical power considering the effect size (% improvement in physiologic function, sample number, and level of significance) was at least 72% in Cohort 1. The results in Cohort 2 showed a significant (p < 0.05) improvement in physiologic functional status of four organs (adrenal glands, hypothalamus and pituitary gland). Average statistical power considering the effect size (% improvement in physiologic function, sample number, and level of significance) was at least 76% in these tests. No significance improvement in cellular physiologic status was observed in pancreas, liver, gall bladder and intestines in Cohort 2. This could be attributed to the fact that discontinue use and not wearing the glutathione patch for 2 days in a week (about 30% less exposure to glutathione) the subjects in Cohort 2 did not have adequate stimulated detoxification in all organs by glutathione over the study period.

More detailed statistical analyses of the EIS data enabled us to make the following observations:

In Cohort 1 (n =10), the average statistical power was more than 72% for all organs showing a highly significant (p < 0.001) improvement in cellular physiologic function. The average statistical power without considering the pituitary gland was more than 82%. The average statistical power, without considering pituitary and intestine was more than 90%.

In Cohort 1 (n = 10), the average statistical power was more than 84% for all organs showing a very significant (p< 0.01) improvement in cellular physiologic function. The average statistical power without considering the pituitary gland was more than 91%. The average statistical power without excluding the pituitary gland and intestine was more than 97%.

In Cohort 1 (n = 10), the average statistical power was more than 91% for all organs showing a significant (p< 0.05) improvement in cellular physiologic function. The average statistical power without considering the pituitary gland was more than 96%. The average statistical power without excluding the pituitary gland and intestine was more than 99%.

In Cohort 2 (n = 20), 4 organs (adrenal glands, hypothalamus and pituitary gland) showed a significant (p< 0.05) improvement in cellular physiologic function.

In summary, the overall data in Cohort 1 demonstrated that the glutathione patch worn 12 hoursdaily over a period of 4 weeks produced a highly significant improvement in physiologic functional status of liver, gall bladder, intestines, adrenals, hypothalamus and pituitary gland and a very significant improvement in pancreas with a statistical power of at least 72%. Stated differently, it could be concluded that the glutathione patch caused a significant improvement in cellular physiologic functional status of pancreas, liver, gall bladder, intestines, adrenals, hypothalamus and pituitary gland with a statistical power > 91%. Therefore, the hypothesis that: The glutathione patch worn 12 hours daily for 4 weeks significantly improves cellular physiologic functional status in different organs was accepted as true.

References:

A. H. Pressman A H. Glutathione: The Ultimate Antioxidant. St. Martin’s Press, New York, NY, 1997.

J. Lyons, A. Rauh-Pfeiffer, Y. M. Yu, X.-M. Lu, D. Zurakowsk, R. G. Tompkins, A. M. Ajamii, V. R. Young and L. Castillo. Blood glutathione synthesis rates in healthy adults receiving a sulfur amino acid-free diet. Proceedings of the National Academy of Sciences, May 9, 2000 vol. 97, No. 10, 5071-5076.

G. Wu, Y. Fang, S. Yang, J. R. Lupton, and N. D. Turner. Glutathione Metabolism and Its Implications for Health. The Journal of Nutrition, 134: 489–492, 2004.

D. M. Townsend, K. D. Tew, H. Tapiero. The importance of glutathione in human disease. Biomedicine & Pharmacotherapy 57, 145–155, 2003.

Haltiwanger, S. A New Way to Increase Glutathione Levels in the Body. Hippocrates Magazine. Vol 28, Issue 1, 48-49, Feb. 2009.

Haltiwanger, S. LifeWave Skin Care Patch Instructions.

Electro Interstitial Scan (EIS) System. http://www.ldtechnologies.com/. Retrieved June 2009.

Bard, A. J.; Faulkner, L. R. “Electrochemical Methods. Fundamentals and Applications” 2nd Ed. Wiley, New York. 2001.

We are Listening to the body Signals! http://www.eisunitedstates.com/. Retrieved June 2009.

LifeWave Patches Research 2009
(IceWave and Glutathione)

Introduction
The following document is a full version of the research project undertaken during April-May 2009 in India at the Centre for bio field
sciences, World Peace Centre, MIT College, Pune.

Study Objective
To determine the efficacy of the LifeWave patches (Ice wave and Glutathione Patches).

Time Period of the Study
April-May 2009

Primary Data Collection
Centre for Biofieldsciences

Aim
The aim of this research project is to examine the changes in energy fields and chakras so as to prove the efficacy of LifeWave patches (Ice Wave and Glutathione Patches).

The following five established devices were chosen to be used for the comparison between before and after scans:

POLYCONTRAST INTERFERENCE PHOTOGRAPHY (PIP)

GAS DISCHARGE VISUALISATION (GDV)

Electro Interstitial Scan (EIS)

Electro Sound level Meter (ESM)

Chakra Temperature

The doctors and researchers working on the project were monitoring all the tests and ensured that the tests, on all the five devices, were carried out perfectly according to the protocol and standard norms & conditions.

Research co-coordinators:

Dr. Thornton Streeter

Dr. Bhagyashri Nilkanth

Dr. Vaibhav Lunkad.

Dr. Anuja Ranade

Dr. Aniruddha Gandhi

Shivali Dandekar

Facilitated by:

Wg.Cdr.A K Zutshi

Sudhir Neurgaoankar

Bapu Sanap

Mrs. Anjali Khamkar

About LifeWave (IceWave and Glutathione)
It’s been known for thousands of years that specific frequencies of light can cause specific changes within the human body. When we go out in the sun, a frequency of light causes our body to make Vitamin D. Another frequency of light (UV) will cause our body to make melanin, the chemical that gives us a sun tan.

LifeWave patches use this knowledge to stimulate acupuncture points on the body for improving the flow of energy without the use of needles and producing drug-free pain relief through the IceWave patches and increased Glutathione levels with the use of the Glutathione patches, within minutes of use.

We can produce an objective assessment through PIP, GDV, EIS, ESM and Chakra Temperature scanning techniques.

Methodology
25 subjects were scanned, who were suffering from any pain for the period of approximately 6 months. Participants were selected randomly through local newspaper advertisements and university notices. Each participant was booked in for two appointments each lasting around 1 hour. They filled their consultation, consent form and then underwent the first round of five scans.

All these participants were then rescanned with the same five devices, again in a random order on second visit.

Visit 1: The before scans were recorded as follows (BASELINE):

PIP (Polycontrast Interference Photography)

GDV (Gas discharge visualization)

Electro Interstitial Scan (EIS)

Electro Sound level Meter (ESM)

Chakra Temperature

After the scans the Patches was applied for a 24 hour period.

Visit 2: PIP, GDV, EIS, ESM, Chakra Temperature repeat/after scanswere done.

For information on the scanning techniques used in this study, please see reference links below:

Polycontrast Interference Photography http://www.biofieldsciences.com/biofieldsciences.com/ PIP.htm

Gas Discharge Visualization http://www.biofieldsciences.com/biofieldsciences.com/ GDV.htm

Electro Interstitial Scan http://www.biofieldsciences.com/biofieldsciences.com/ EIS.htm

Total No. of subjects	25
Control	5
Experimental	10 IceWave
	10 Glutathione
Age	21-70 yrs
Sex	male or female

Scans were carried out and analyzed by leading experts in the specific imaging technology. Most of the results were qualitative and cross comparison was complex. Overall there were significant positive changes in all 5 assessment techniques. (See Appendix for case specific examples). The participants were then randomly rescanned with the same five devices.

Results
Scans were obtained of 25 participants. Each case was individually examined and analyzed. The results obtained support the effectiveness of the Life Wave Patches.

PIP scans showed positive change in 18 participants out of 20.

Two (2) of these 20 scans showed no changes.

The GDV, EIS, ESM and Chakra temperature scans show results in correlation with PIP.

Conclusion
It seems clear that the LifeWave- ICEWAVE Patches (have a profound effect on pain management and LifeWave –Glutathione patches have a profound effect in detoxification. In addition to the analysis part of the data, during experiments changes witnessed in the biofield were strongly positive in the after scans. From this research we can conclude that the LifeWave Patches (ICEWAVE AND GLUTATHIONE) are a very effective means of neutralizing pain and in detoxification as well.

Maximum changes were seen in the solar plexus and lower back along with significant positive change in the biofield.

Predominance of harmonious green and pink frequencies increase with prolonged use of the Life Wave Patches (ICEWAVE AND GLUTATHIONE).

Blocked chakras, especially solar plexus, are seen to open up. Red congested energy is replaced by green or violet healing energy.

Positive effects are also seen over lung fields in some of the cases.

Muscle spasm were reduced after applying IceWave patch.

These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease. The information provided on this website and in emails is for educational purposes only and is not intended as a substitute for advice from your physician or other health care professional. You should not use the information on this website and emails to diagnose or treat any health problems or illnesses without first consulting with your doctor. You should consult with a healthcare professional before starting any diet, exercise or supplementation program, before taking any medication, or if you have or suspect you might have a health problem.

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